Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk

Publication Type

Journal contribution (peer reviewed)

Authors

von Neubeck, M.; Huptas, C.; Glück, C.; Krewinkel, M.; Stoeckel, M.; Stressler, T.; Fischer, L.; Hinrichs, J.; Scherer, S.; Wenning, M.

Year of publication

2016

Published in

International Journal of Systematic and Evolutionary Microbiology

DOI

10.1099/ijsem.0.000852

Page (from - to)

1163-1173

Analysis of raw cow's milk and semifinished milk products microbiota yielded seven isolates assigned to the genus Pseudomonas which formed two individual groups in a phylogenetic analysis based on partial rpoD and 16S rRNA gene sequences. The two groups could be differentiated from each other and also from their closest relatives as well as from the type species Pseudomonas aeruginosa by phenotypic and chemotaxonomic characterisation and ANIb values calculated from draft genome assemblies. ANIb values within the groups are higher than 97.3 %, whereas similarity values to the closest relatives are equal or lower than 85 %. The major cellular lipids of the type strains WS4917T and WS4993T are phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG); the major quinone is Q-9 in both strains with small amounts of Q-8 in strain WS4917T. The DNA G+C content of the strains WS4917T and WS4993T were 58.08 % and 57.30 %, respectively. Based on these data the type strain WS4917T (= DSM 29165T = LMG 28433T) and the additional strains WS4995 (= DSM 29141 = LMG 28434), WS4999, WS5001 and WS5002 should be considered as a novel species of the genus Pseudomonas for which the name Pseudomonas helleri sp. nov. is proposed. The type strain WS4993T (= DSM 29166T = LMG 28437T) and the additional strain WS4994 (= DSM 29140 = LMG 28438) should also be recognized as a novel species of the genus Pseudomonas for which the name Pseudomonas weihenstephanensis sp. nov. is proposed.