Bioreactor cultivation of Escherichia coli for production of recombinant penicillin G amidase from Alcaligenes faecalis

Publikations-Art

Zeitschriftenbeitrag (peer-reviewed)

Autoren

Deak, P. M., Lutz-Wahl, S., Bothe, H., Fischer, L.

Erscheinungsjahr

2003

Veröffentlicht in

Biotechnology Letters

Band/Volume

25/5

DOI

10.1023/A:1022498314354

Seite (von - bis)

397-400

The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.