Biotransformation of nitriles to amidases using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4

Publikations-Art

Zeitschriftenbeitrag (peer-reviewed)

Autoren

Kubac, D., Kaplan, O., Elisakova, V., Patek, M., Vejoda, V., Slamova, K., Tothova, A., Lemaire, M., Gallienne, E., Lutz-Wahl, S., Fischer, L., Kuzma, M., Pelantova, H., van Pelt, S., Bolte, J., Kren, V., Martinkova, L.

Erscheinungsjahr

2008

Veröffentlicht in

Journal of Molecular Catalysis

Verlag

Elsevier

Serie/Bezeichnung

B: Enzymatic

DOI

10.1016/j.molcatb.2007.09.007

Seite (von - bis)

107 - 113

A semi-purified nitrile hydratase from Rhodococcus erythropolis A4 was applied to biotransformations of 3-oxonitriles, 3-hydroxy-2-hethylenenitriles, 4-hydroxy-2-methylenenitriles, 3-hydroxynitriles and 3-acyloxynitrile into amides. Crosslinked enzyme aggregates (CLEAs) with nitrile hydratase and amidase activities (88% and 77% of the initial activities, respectively) were prepared from cell-free extract of this microorganism and used for nitrile hydration in presence of ammonium sulfate, which selectively inhibited amidase activity. The genes nha1 and nha2 coding for alpha and beta subunits of nitrile hydratase were cloned and sequenced.