Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk

Publikations-Art

Zeitschriftenbeitrag (peer-reviewed)

Autoren

Von Neubeck, M.; Huptas, C.; Glück, C.; Krewinkel, M.; Stoeckel, M.; Stressler, T.; Fischer, L.; Hinrichs, J.; Scherer, S.; Wenning, M.

Erscheinungsjahr

2017

Veröffentlicht in

International Journal of Systematic and Evolutionary

DOI

10.1099/ijsem.0.001836

Five strains, designated WS 4672T, WS 4998, WS 4992T, WS 4997, and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T, and Pseudomonas libanensis CIP 105460T showing 99.7-99.6 % similarity. Using rpoD gene sequences Pseudomonas veronii LMG 17761T (93.3 %) was most closely related to strain WS 4672T and Pseudomonas libanensis CIP 105460T to strain WS 4992T (93.3 %). The five strains could be differentiated from their closest relatives and from each other by phenotypic and chemotaxonomic characterization and ANIb values calculated from draft genome assemblies. ANIb values of WS 4992T and WS4671T to the closest relatives are lower than 90 %. The major cellular polar lipids of both strains are phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phospholipid (PL) and diphosphatidylglycerol (DPG); their major quinone is Q-9. The DNA G+C content of strains WS 4992T and WS 4672T were 60.0 % and 59.7 %, respectively. Based on these genotypic and phenotypic traits the type strain WS 4992T (= DSM 29167T = LMG 28435T) and the additional strains WS 4997 and WS 5000 should be considered to represent a novel Pseudomonas species for which the name Pseudomonas lactis is proposed. The strains WS 4672T and WS 4998 should also be recognized as a novel Pseudomonas species for which the name Pseudomonas paralactis is proposed. The type strain is WS 4672T (= DSM 29164T = LMG 28439T).